MPSC Assistant Chemical Analyser Syllabus 2026 - Check Group-B Exam Pattern

The Maharashtra Public Service Commission (MPSC) has released the syllabus for the post of Assistant Chemical Analyser under the Directorate of Forensic Science Laboratories (DFSL), General State Services, Group-B, Home Department, Government of Maharashtra. This article covers the complete subject-wise syllabus for the post, based on the official syllabus document. Candidates preparing for this exam should go through every topic carefully, as the syllabus spans Analytical Chemistry, Inorganic Chemistry, Organic Chemistry, and Biochemistry techniques.

MPSC Assistant Chemical Analyser Syllabus and Exam Pattern 2026 - Overview

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MPSC Assistant Chemical Analyser Syllabus 2026

The syllabus for Assistant Chemical Analyser is divided into four major units covering analytical chemistry fundamentals and its application across inorganic, organic, and biochemical sample analysis. The detailed topic-wise breakdown is given below.

1. Analytical Chemistry

(A) Basic Aspects

• Sampling of solid, liquid, and gas samples
• Molecular and equivalent weight
• Molarity, molality, normality
• Solution preparation in w/w, w/v, and v/v terms
• Mole concept, concentrations, ppm, ppb, and ppt
• Calibration and validation: calibration curve, regression analysis, external standard, internal standard, and standard addition method

(B) Statistical Treatment of Results

• Accuracy, precision, sensitivity, detection limit
• Errors and their types, mean, median, standard deviation, relative standard deviation
• Gaussian curve, t-test, F-test, confidence interval
• Criteria for selection of analytical methods: accuracy, precision, sensitivity, selectivity, specificity, detection limit

(C) Titrimetry

• Types of titrations: acid-base, complexometric, redox, precipitation
• Standards used for standardisation: primary standard and secondary standard
• Potentiometric and conductometric titration and their applications

(D) Gravimetric and Thermogravimetric Analysis

• Precipitation and different precipitants used in gravimetric analysis
• pH requirement for precipitation
• Quantitative analysis
• Thermogravimetric analysis
• Mass spectrometry for qualitative and quantitative analysis, and related problems

(E) Separation Techniques

• Extraction methods
• Distillation methods: vacuum distillation, steam distillation, fractional distillation
• Purification and identification of substances

2. Analytical Techniques in Inorganic Chemistry for Different Inorganic Samples

(A) Spectroscopy and Chromatography for Inorganic Sample Analysis

• Atomic absorption, atomic emission, and inductively coupled plasma-atomic emission and mass spectrometry (ICP-AES and ICP-MS)
• UV-Visible spectroscopy for qualitative and quantitative analysis, Job's Method, and applications
• Ion exchange chromatography for analysis of various inorganic cations and anions

(B) Microscopy for Inorganic Sample Analysis

• Trace and heavy metal, glass, soil, and liquid sample analysis using Scanning Electron Microscopy (SEM)
• Energy Dispersive Spectroscopy (EDS)

(C) Electroanalytical Techniques

• Coulometry and its types
• Amperometry, voltammetry, cyclic voltammetry, stripping voltammetry
• Polarography

3. Analytical Techniques in Organic Chemistry

(A) Spectroscopy

• Theory and application of UV-Visible, IR (FTIR), Raman, NMR, and Mass spectrometry
• Interpretation of spectra and structure elucidation

(B) Separation Techniques

• Theory and application in chemical analysis of TLC, HPTLC, GC, GC-MS, LC-MS, HRMS, column chromatography, and paper chromatography
• HPLC: ion exchange chromatography, adsorption chromatography, normal and reverse phase chromatography, solid phase extraction

(C) Organic Analysis

• Pharmaceutical and narcotic drugs, psychotropic substances
• Alcohol estimation
• Petroleum products, paints and dyes, rubber, plastic, fibres
• Organic poisons such as insecticides, pesticides, herbicides, etc.

4. Analytical Techniques in Biochemistry

(A) Biomolecules

• Structure, functions, and estimation of carbohydrates, lipids, proteins, nucleic acids
• Biological buffers

(B) Molecular Biology

• DNA isolation, DNA quantification
• PCR, RT-PCR, RFLP (Restriction Fragment Length Polymorphism)
• ELISA, RIA (Radio Immuno Assay)
• Antigen-antibody reactions

(C) Separation Techniques

• SDS-PAGE, one dimension (PAGE) and two dimension electrophoresis
• Isoelectric focusing
• Gel filtration chromatography, affinity chromatography
• High voltage electrophoresis, agarose electrophoresis, capillary electrophoresis

(D) Isotope Tracer Technique

• Basic concepts and principles
• Advantages, limitations, and applications in biochemistry

(E) Enzymes

• Enzyme classification, isoenzymes
• DNA polymerase
• Enzyme kinetics (basic)

(F) Blood and Other Body Fluid and Hair Examination (Human and Animal)

• Blood group system
• Semen examination, saliva, urine, hair, sweat
• Biological stains and their preservation

How to Prepare for MPSC Assistant Chemical Analyser Exam 2026

• Start with the four core units and build a subject-wise study timetable, giving more time to Analytical Chemistry and Organic Chemistry techniques since they form the base for the other two units.
• Focus on instrumental technique names, their principles, and applications (e.g., ICP-MS, GC-MS, HPLC, SEM-EDS) since spectroscopy and chromatography questions are commonly asked in analytical chemistry exams of this type.
• Revise statistical treatment of results (t-test, F-test, standard deviation, confidence interval) separately, as this topic is calculation-based and needs practice.
• Make short notes on separation and electrophoresis techniques used in biochemistry, since these topics overlap with forensic biology concepts.
• Practice previous year question papers of similar forensic/analytical chemistry posts (where available) to understand question difficulty and pattern.
• Keep checking the official MPSC website regularly for the release of the detailed exam pattern, marks distribution, and exam date.

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MPSC Assistant Chemical Analyser Syllabus 2026 - Important Links